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Table 2 Transformation frequencies using Himar1 constructs.

From: Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations

Plasmid

    Mean transformation frequency (transformants/CFU) [SE]

Experiment 1

Experiment 2

pMHWT-2

0

NT

pMHWT-1

2.5 × 10-6 [1.3 × 10-6]

9.0 × 10-8 [2.4 × 10-8]

pMHC9-1

9.3 × 10-6 [4.5 × 10-6]

3.0 × 10-7 [4.6 × 10-8]

  1. Mean transformation frequencies (with standard errors) are shown for three separate transformations of M. hyopneumoniae strain 232 using plasmid constructs pMHWT-1, pMHWT-2 and pMHC9-1 in two separate experiments. In experiment 1, 106 CFU were transformed with each plasmid while in experiment 2, 108 CFU were transformed.
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Veterinary Research

ISSN: 1297-9716