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Figure 4 | Veterinary Research

Figure 4

From: Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations

Figure 4

Southern analysis of mutant M. hyopneumoniae. Total DNA was extracted from 11 individual transformants of M. hyopneumoniae 232 generated using plasmid pMHC9-1, and subjected to Southern analysis using a probe specific for tetM. Single bands were generated for 10 of the mutants suggesting single transposon insertion sites. Two separate bands were present for mutant 1. No band was present for the untransformed M. hyopneumoniae strain 232 negative control (NC) and an intense band was present for the pMHC9-1 positive control (PC) as expected.

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Veterinary Research

ISSN: 1297-9716