Skip to main content
Figure 4 | Veterinary Research

Figure 4

From: Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection

Figure 4

Impact of O. ostertagi ESP on parietal cell activity. (A) Cultured rabbit parietal cells were pre-incubated with parasite ESP at different concentrations for 24 h, and then stimulated with histamine (10–5 M) or carbachol (10–5 M) for 15 min. Quantitation of acid-secretion was measured using 9-aminoacridine accumulation as described in the material and methods. Data are expressed as percentages (means ± SEM) of secretory index in histamine or carbachol stimulated parietal cells in the absence of ESP treatment. (B) Quantification of acid secretion in histamine stimulated rabbit parietal cells following 24 h of pre-incubation with native adult extract, adult ESP and boiled ESP at 100 μg/mL (C) Quantification of acid secretion following 15 min and 24 h of incubation with adult ESP. (D) Quantification of acid secretion in dbcAMP stimulated rabbit parietal cells following 24 h of preincubation with 100 μg/mL adult ESP. Data are expressed as percentages (means ± SEM) of secretory index in dbcAMP or carbachol stimulated parietal cells in the absence of ESP treatment. (**p value ≤ 0.01, ***p value ≤ 0.001).

Back to article page