Figure 5

Cell death and histopathologic analysis of MLN from Salmonella Typhimurium infected pigs. A-D: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analysis at 0 (A), 1(B), 2 (C) and 6 (D) dpi. Scale bar = 100 μm. E-H: H&E staining of at 0 (E), 1(F), 2 (G) and 6 (H) dpi. Scale bar = 50 μm. I: Quantification of TUNEL fluorescent labeling shows an increase of positive nuclei at 1 dpi and a decrease to levels inferior than the controls at 2 and 6 dpi. J-K: mRNA quantification of CASP1 (J) and CASP3 (K) by qPCR.