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Figure 2 | Veterinary Research

Figure 2

From: Evolution of foot-and-mouth disease virus intra-sample sequence diversity during serial transmission in bovine hosts

Figure 2

Quantification of viral RNA copy number and clinical signs (temperature) of infected hosts. FMDV RNA load in samples collected during the serial passage of FMDV through four calves, detected by real-time reverse-transcription polymerase chain reaction (rRT-PCR). Graph A–D, calf 1, 2, 3 and 5 (A1, A2, A3 and A5) respectively. A (A1) previously discussed in [29], sequenced samples in white with thick border and non-sequenced samples in white; B-D (A2, A3 and A5), sequenced samples in dark gray with thick border and non-sequenced samples in light gray. Inoculum (Inoc [A1 only]); serum (SR); probang (PB); front left foot (FLF) lesion; front right foot (FRF) lesion; back left foot (BLF) lesion; back right foot (BRF) lesion. Dashed lines indicate the minimum initial viral load to be amplified and then sequenced (106 copies of FMDV RNA/μL of sample) for A2, A3 and A5. Gray arrows indicate the time the calf spent in contact with the next calf, while black arrows indicate the time spent in contact with the previous calf on the transmission chain. Animal temperatures are shown on the same graphs (black solid line). White stars indicates the day when the first foot lesions appeared (FRF and BLF for both A2 and A3), while black stars indicate the day at which lesions appeared on all four feet.

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