Influence of LPS on the localization of β-casein and adipophilin in AEC. Mammary glands non-treated (0 h) and at 3, 6, and 12 h after LPS injection were immunostained with anti-β-casein (red) and anti-adipophilin (green) antibodies. Immunostained images of β-casein (A) and adipophilin (B) were merged with bright field images and DAPI stained images (blue). Beta-casein-negative AEC were observed 12 h after LPS injection (arrowhead). Adipophilin staining shows the shape of lipid droplets in AEC. Scale bars: 20 μm.