LPS influence on the localization of AQP3 and GLUT-1 in AEC. Mammary glands non-treated (0 h) and at 3, 6, and 12 h after LPS injection were immunostained with anti-AQP3 and anti-GLUT-1 antibodies. (A) Immunostained images of AQP3 (green) were merged with bright field images. (B) Immunostained images of GLUT-1 (green) were merged with pan-keratin (red) as a marker of epithelial cells. Blue represents nuclei stained with DAPI. Scale bars: 20 μm.