qPCR validation of IVL , FLG and LOR expression in ovine skin following P. ovis infestation. Expression data across six individual sheep for IVL (a) FLG (b) and LOR (c). Values for IVL and FLG were normalised to the expression of the ovine housekeeping gene GAPDH and expressed as copies/μL at each time point across six animals. LOR gene expression was assessed using the relative quantification ddCt method and normalised to the time = 0 sample. * = p < 0.05, ** = p < 0.01, *** = p < 0.001. Error bars show standard error of the mean (SEM).