Characterization of NCR1+ cells residing in lymphoid tissues in the gut of lambs indicates that the majority are NK cells

Table 1 Primary antibodies used in this study

Antibody	Clone	Isotype	Specificity	Cellular expression	Source	Used in	Cross reactivity^a	References
Mouse anti-bovine NCR1	AKS4	IgG1	NCR1	Natural killer cells		Flow	Sheep, goat	[26–28]
Mouse anti-bovine NCR1	AKS6	IgG2b	NCR1	Natural killer cells		Flow	Sheep, goat	[26–28]
Rat anti-human CD3: Pacific blue (MCA1477)	CD3-12	IgG1	CD3ϵ	All T cells	AbD Serotec, Ltd., Oxford, UK	Flow	Cattle, pig, horse, dog, cat and other mammals
Mouse anti-human CD16	KD1	IgG2a	CD16	NK cells, macrophages, some T cells		Flow	Sheep, cattle	[5, 23]
Mouse anti-ovine NCR1	EC1.1	IgG1	NCR1	Natural killer cells		IF		[24]
Rabbit anti-human CD3 (A0452)		Poly-clonal	CD3ϵ	All T cells	Dako, Glostrup, Denmark	IF	Sheep, goat, cattle and others	[29]
Mouse anti-bovine CD205 (MCA1651)	CC98	IgG2b	CD205	Dendritic cells, some T cells, some B cells, some epithelial cells	AbD Serotec, Ltd., Oxford, UK	IF	Sheep
Mouse anti-bovine CD11c (BAQ153A)	BAQ153A	IgM	CD11c	Dendritic cells, some macrophages	VMRD, Inc., WA, USA	IF	Sheep, goat, cattle and others
Rabbit anti-Ki67 (ab15580)		Poly-clonal	Ki67	Proliferating cells	Abcam, Cambridge, UK	IF	Cattle, horse, dog, mouse, rat and other mammals

ISSN: 1297-9716