Effect of vaccination of cattle with the low virulence Nc-Spain 1H isolate of Neospora caninum against a heterologous challenge in early and mid-gestation

Table 1 Summary of groups

Group^a	Immunisation	Challenge time	Challenge dose	Number of animals
Group^a	(Nc-Spain 1H tachyzoites)^b	(days of gestation)	(Nc-1)	Number of animals
1 (Immunised/non-challenged)	10⁷	-	-	5
2 (Immunised/70-challenged)	10⁷	70	10⁷	5
3 (Non-immunised/70-challenged)	None	70	10⁷	5
4 (Immunised/135-challenged)	10⁷	135	4 × 10⁸	5
5 (Non-immunised/135-challenged)	None	135	4 × 10⁸	4
6 (Non-immunised/non-challenged)	None	-	-	3
7 (Immunised/non-challenged)	10⁷	-	-	11
8 (Non-immunised/non-challenged)	None	-	-	10

^a Groups 1 to 6 were maintained at the experimental farm (UCM), whereas groups 7 and 8 were kept at the dairy farm throughout the experiment.
^b Heifers were subcutaneously immunised twice at 4-week intervals prior to artificial insemination.

ISSN: 1297-9716