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Table 1 Brucella strains used.

From: Deletion of the GI-2 integrase and the wbkA flanking transposase improves the stability of Brucella melitensis Rev 1 vaccine

Strain Characteristics Source/ reference
B. melitensis H38 B. melitensis smooth virulent strain; S-LPS CITA collection
H38-NxR B. melitensis H38 spontaneous mutant resistant to nalidixic acid; challenge studies in mice; S-LPS This work
Rev 1 B. melitensis smooth attenuated reference vaccine strain; S-LPS CITA collection
Rev 1ΔwbkA Rev 1 spontaneous deletion mutant in wbkA (ORF BMEI1398 and BME1412); R-LPS This work
Rev 1ΔGI-2 Rev 1 spontaneous deletion mutant in GI-2; R-LPS This work
Rev 1ΔISBm1 Rev 1 in-frame deletion mutant in ISBm-1 transposase gene (ORF BMEI1398 and BME1400); S-LPS This work
Rev 1Δint Rev 1 in-frame deletion mutant in GI-2 phage-integrase gene (nucleotides intΔ53–286); S-LPS This work
Rev 2(ΔISBm1 Δint) Rev 1 double in-frame deletion mutant in ISBm-1 transposase and GI-2 phage-integrase; S-LPS This work
Rev 1ΔwbkA/c Rev 1ΔwbkA rough spontaneous mutant complemented with plasmid pMM14; S-LPS This work
Rev 1ΔGI-2/wboAB Rev 1ΔGI-2 rough spontaneous mutant containing the plasmid pMM76; R-LPS This work
Rev 1ΔGI-2/c Rev 1ΔGI-2 rough spontaneous mutant complemented with plasmid pBGI-997-99c; S-LPS This work
  1. S-LPS: smooth lipopolysaccharide; CITA: Centro de Investigación y Tecnología Agroalimentaria of Aragón (Zaragoza, Spain); R-LPS: rough lipopolysaccharide; GI-2: Genomic Island 2.