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Figure 1 | Veterinary Research

Figure 1

From: Deletion of the GI-2 integrase and the wbkA flanking transposase improves the stability of Brucella melitensis Rev 1 vaccine

Figure 1

Genetic organization of regions encoding LPS genes that are susceptible to mutation with a subsequent generation of R mutants. (A) GI-2 region of B. melitensis and the scar and circular intermediate (wbo genes are omitted in the circular intermediate) resulting from the int- promoted excision; (B) wbkA region and the corresponding scar and circular intermediate resulting from homologous recombination between ISBm1 transposases; (C) the wbk FD region of B. melitensis and B. canis (wbkD is annotated as capD in B. canis); (D) wbo locus in B. melitensis and B. abortus RB51 and (E) manBAcore locus in B. melitensis chromosome II with its corresponding mutants showing the deletion and point mutations in manBcore gene (asterisks). The position of the primers used in the PCR analyses is indicated as P-number of the primer. The ORF annotations are based on the B. melitensis 16 M genome [GenBank: AE008917 and AE008918] and B. canis chromosome I [GenBank: CP000872].

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