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Figure 2 | Veterinary Research

Figure 2

From: A novel porcine reproductive and respiratory syndrome virus vector system that stably expresses enhanced green fluorescent protein as a separate transcription unit

Figure 2

Characterisation of recombinant rHP-PRRSV/SD16/TRS6-EGFP containing an encoding EGFP as a separate transcription unit. A. Marc-145 cells infected with rHP-PRRSV/SD16/TRS6-EGFP and HP-PRRSV/SD16 were observed for CPE and fluorescence detection, respectively. Living cells were analysed by phase contrast and fluorescence microscopy. B. Western blot analysis of virus N protein and EGFP levels in Marc-145 cells infected with wild-type HP-PRRSV/SD16 or recombinant rHP-PRRSV/SD16/TRS6-EGFP.

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