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Figure 4 | Veterinary Research

Figure 4

From: Two major ruminant acute phase proteins, haptoglobin and serum amyloid A, as serum biomarkers during active sheep scab infestation

Figure 4

Relationship between lesion area and serum SAA during a 6 week infestation of sheep with Psoroptes ovis. Panel a: Serum from a sheep infested with P. ovis in trial TCT where the lesion resolved naturally after 4 weeks (Sheep 1); Panel b: Serum from an infested sheep in the same trial where the lesion had continued to expand during the 6 week time course (Sheep 6). The blots were probed with an anti-SAA antibody raised in rabbits against human rSAA (Abcam) and the secondary antibody conjugate was swine anti-rabbit HRP IgG (Dako). The blot was visualised using ECL Plus (GE Healthcare) and ImageQuant. Band volumes (at 14 kDa) were quantified by ImageQuant TL software.

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