Figure 7

Amino acid sequence alignments of three major genes among ADRV and other ranaviruses. (A) Multiple alignments of the deduced two duplicate US22 family-like proteins in ADRV (6R, 49L) and RGV (6R, 106R). The secondary structure of US22 domain that is comprised of four conserved α-helices (red H) and six β-strands (blue E) was shown above the alignments, and the completely conserved residues are indicated by black shaded regions. (B) Multiple alignments of ADRV 84L and its homologues in other known ranaviruses, such as TFV, ATV, CMTV, RGV and FV3. The N-terminal PKR-binding domain (S1), central helical domain (HD) and C-terminal domain (CTD) are shown above the alignments, and two cysteines are indicated by green. The VXRVDXXKGYXDL motif is marked by a box. The black shaded regions indicate completely conserved residues, while the grey shaded regions are partially conserved residues with greater than 80% identity. (C) Multiple alignments of ADRV 75L and its homologues in RGV, CMTV and FV3. The homologous regions for ADRV 75L in CMTV (nucleotides 77077–77352) and FV3 (nucleotides 41633–41908) were translated into amino acid sequences and used in the alignments. The conserved residues are indicated by black shaded regions. The nuclear localization signal (NLS) motif is marked by the purple box, and the nuclear export signal (NES) motif by the blue box.