Infectivity and transmissibility of H9N2 avian influenza virus in chickens and wild terrestrial birds

Table 2 Number of infected and contact uninfected naïve birds showing the presence of virus in buccal and cloacal swabs collected over 29 days

Species	Infection route	Number of birds	Passage number	Swab samples	Days post infection
Species	Infection route	Number of birds	Passage number	Swab samples	2	3	4	5	6	7	9	11	15	19	23	27	29
Broilers	Infected	10	1^st	B	5	10	10	10	10	10	10	9	2	-	-	-	-
	Infected	10	1^st	C	-	10	10	10	4	4	-	-	-	-	-	-	-
	Contact	5	1 ^st	B	2	3	3	4	4	4	4	4	-	-	-	-	-
	Contact	5	1 ^st	C	-	5	5	5	1	-	-	-	-	-	-	-	-
Layers	Infected	10	1^st	B	7	10	10	10	10	10	10	10	-	-	-	-	-
	Infected	10	1^st	C	-	10	10	10	-	-	-	-	-	-	-	-	-
	Contact	5	1 ^st	B	1	4	3	4	4	4	4	2	-	-	-	-	-
	Contact	5	1 ^st	C	-	5	5	4	2	-	-	-	-	-	-	-	-
Jungle fowl	Infected	10	1^st	B	7	10	10	10	10	10	10	10	1	- -	- -	- -	- --
	Infected	10	1^st	C	-	10	10	10	6	3	-	-	-	- -	- -	- -	- --
	Contact	5	1 ^st	B	3	2	2	2	2	2	1	1	-	- -	- -	- -	- -
	Contact	5	1 ^st	C	-	5	5	5	4	-	-	-	-	- -	- -	- -	- -
Quail	Infected	10*	1^st	B	10	10	8	8	8	8	6	-	-	- -	- -	- -	- -
	Infected	10*	1^st	C	-	10	8	8	7	1	-	-	-	- -	- -	- -	- -
	Contact	5	1 ^st	B	4	5	5	4	4	2	-	-	- -	- -	- -	- -	- -
	Contact	5	1 ^st	C	-	5	5	5	5	2	-	-	- -	- -	- -	- -	- -
Sparrows	Infected	10	1^st	B	10	10	10	10	10	10	3	-	-	-	-	-	-
	Infected	10	1^st	C	-	10	10	10	6	-	-	-	-	-	-	-	-
	Contact	5	1 ^st	B	5	5	4	4	4	3	-	-	-	-	-	-	-
	Contact	5	1 ^st	C	-	5	5	5	4	-	-	-	-	-	-	-	-
Crows*	Infected	10	1^st	B	-	-	-	-	-	-	-	-	-	-	-	-	-
			1^st	C	-	-	-	-	-	-	-	-	-	-	-	-	-
			2^nd	B	1	9	9	10	10	8	-	-	-	-	-	-	-
			2^nd	C	-	10	10	10	6	1	-	-	-	-	-	-	-
	Contact	5	1 ^st	B	-	-	-	-	-	-	-	-	-	-	-	-	-
			1 ^st	C	-	-	-	-	-	-	-	-	-	-	-	-	-
			2 ^nd	B	-	-	-	-	-	-	-	-	-	-	-	-	-
			2 ^nd	C	-	-	-	-	-	-	-	-	-	-	-	-	-

*swabs from crows only showed virus presence after two serial passages in eggs. B, denotes buccal swabs. C, denotes cloacal swabs and -, indicates that swabs were negative for virus. Infected birds were inoculated with 10^6.6 EID₅₀ UDL-01/08 virus. Swab samples were inoculated into EHE and virus in the harvested allantoic fluid was detected by HA assay. * two quail on 4 dpi died and from this time point onward swabs from 8 birds were analysed. Contact birds are indicated in bold.

ISSN: 1297-9716