Modulation of the host Th1 immune response in pigeon protozoal encephalitis caused by Sarcocystis calchasi

Table 2 Sequences of primers used for RT-qPCR and uncorrected ( p ) sequence distance of obtained pigeon amplicons to chicken mRNA

RNA target	5^′-3^′ primer sequences	Amplicon size (bp)	Primer efficiency (%)	p-distance*	Accession number**
beta-actin	F: AAGGACCTGTACGCCAACAC	211	91.8	0.024	NM_205518
	R: CCTGCTTGCTGATCCACATC
GUSB	F: GGGGCAAACTCCTTCCG	223	92.2	0.127	NM_001039316
	R: ATCCACCAGCTTGATGTCACTAAC
HMBS	F: CTGGCCCGGATTCAGAC	154	96.3	0.166	XM_417846
	R: GCTCTTTGGTGAAGAGGCTC
RPL13	F: CCACAAGGACTGGCAGCG	135	92.0	0.095	NM_204999
	R: ACGATGGGCCGGATGG
TFRC	F: GCCCTGAATGACAGGATGATG	206	87.3	0.17	NM_205256
	R: GTCCACGTCGCTAGGGCC
IL-1	F: CGAGAGCAGCTACGCCG	271	99.6	0.176	DQ393270
	R: GCCGCTCAGCACACACG
IL-6	F: CTGCCCAAGGTGACGGAG	178	97.9	0.3	HM179640
	R: CCAGGTGCTTTGTGCTGTAGC
IL-7	F: CAGAGTATCGTGACAGATGCTGC	174	101.3	0.111	NM_001037833
	R: ATGAGACTAATGCTGCTTTCCTTC
IL-8	F: CAAGACGTGAAGCTGACACAGAG	161	99.5	0.117	DQ393275
	R: GGTGCATCAGAATTGAGTTGAG
IL-12	F: AGTGAAGGAGTTCCCAGATGC	188	90.0	0.194	DQ202328
	R: TTCCAGAGTAGTTCTTTGCCTCAC
IL-15	F: GAATGCCAGGAACCTGTAATG	246	102.5	0.142	HQ005358
	R: GCATTCCCTCTGTATAACCTTTAC
IL-18	F: GCCAGTTGCTTGTGGTTCG	160	100.8	0.141	AY775782
	R: TCTACCTGGACGCTGAATGC
IFN-γ	F: CAGATGTAGCTGACGGTGGAC	276	93.2	0.178	DQ479967
	R: GCTCATGCACAGCTTTGCG
TGF-β2	F: GAAGAAGCGTGCTCTAGATGC	105	100.1	0.019	ENSGALT00000015664
	R: CATTTCCAGCCAAGATCCC
LITAF	F: CCCATCTGCACCACCTTCAT	157	100.8	0.252	NM_204267
	R: TGCTGCACATACACAGTCTGAAC
TL1A	F: CCTGAGTTATTCCAGCAACGCA	285	95.3	0.111	NM_001024578
	R: ATCCACCAGCTTGATGTCACTAAC

ISSN: 1297-9716