Figure 6

TEM of poBMDC co-infected with H3N2 SwIV and H. parasuis at 8 hpi. a, b and c: H3N2 SwIV plus SW114 strain. a: Several bacteria were observed inside the poBMDC (white-arrow) occupying phagolysosomes, and few vesicles, Bar = 2 μm. b: Many SW114 bacteria were observed attached to the cellular membrane (white-arrow), as can be observed in the close-up (left-up box), Bar = 1 μm. c: In some phagolysosomes which contained bacteria (white-arrow) also virus-like particles (VLP) were observed, (black-arrow) 100 nm in diameter, Bar = 0.5 μm. d, e and f: H3N2 SwIV plus Nagasaki strain. d: Few Nagasaki bacteria (white-arrow) were seen in poBMDC. However, many vesicles of different sizes which occupied large sub-cellular space were observed; less organelles were seen, Bar = 2 μm. e: Some poBMDC showed a large number of bacteria (white-arrow) inside and outside. Nagasaki was also, to a lesser extent, attached to the cellular membrane, Bar = 1 μm. f: This figure shows a phagolysosome-like structure with Nagasaki strain and VLP (black-arrow) inside, in the upper-left box VLP can be observed. Bar = 0.2 μm.