Figure 5

TEM of mock poBMDC or H. parasuis infected-poBMDC at 1hpi (a,b,c) and at 8 hpi (d,e,f). a and d: Mock poBMDC with normal morphology of DC, dendritic processes (Dp) as well as some organelles such as mitochondria (mit). b and e: SW114-infected- poBMDC, b: poBMDC with several vesicles in the cytoplasm (ves) some of them containing bacteria (white-arrows indicate some of the bacteria). Bacteria were inside phagolysosomes and some had a degenerated aspect (more electron dense); in the upper-left box, details of bacteria can be observed bounded to phagosome membrane. e: 8 h later, more SW114 bacteria were appreciated inside poBMDC (white-arrows and close-up). c and f: Nagasaki- infected- DCs. c: 1 hpi, there were several vesicles (ves) in the cytoplasm, of different sizes and, some contained bacteria (white-arrow) as can be noted in the upper-left box. f: 8h later, the number and size of vesicles (ves) increased and also the Nagasaki bacteria were found inside vesicles (white-arrow) within the cell (close-up). Bar = 2 μm.