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Figure 1 | Veterinary Research

Figure 1

From: Differential interactions of virulent and non-virulent H. parasuis strains with naïve or swine influenza virus pre-infected dendritic cells

Figure 1

Experimental design. (a) H. parasuis single infection. At day 8 of differentiation, poBMDC were infected with SW114 or Nagasaki. Infected cells were collected at 1h and 8 hpi for IF, ICS or fixed for TEM studies. At 8 hpi, supernatants were collected for cytokine screening by ELISA. (b) Co-infection of poBMDC with H3N2 SwIV and H. parasuis. Porcine BMDC were infected with H3N2 SwIV for 1h followed by H. parasuis for another 1h. After that cells were left for a further 8h to obtain supernatants for ELISA. Cells for IF, ICS and TEM were collected at 1h and 8 hpi. (c) Intracellular survival assay. After 1h of co-infection (time zero), the cells were washed and incubated for a further 2 or 4h with medium containing gentamicin and penicillin G. At t0, t2 and t4, cells were disrupted with water to quantify the CFU/mL. IF- immunofluorecence, ICS- intracellular staining, TEM- transmission electron microscopy.

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