Figure 1From: Plasmid DNA immunization with Trypanosoma cruzi genes induces cardiac and clinical protection against Chagas disease in the canine modelrSSP4 and rSP protein expression in the HeLa plasmid transfected cells. (A) SDS-PAGE and (B) western blot analysis of the pBCSSP4 plasmid transfected cell extracts; samples were taken at different time points; Lanes: 2 and 3, 8 h; 4 and 5, 24 h; 6 and 7, 48 h; 8 and 9, 56 h. The membrane was probed with the anti-MBP::SSP4 antibody. (C) SDS-PAGE and (D) western blot analysis of the pBCSP plasmid transfected cell extracts; samples were taken at different time points; Lanes: MWM, molecular weight marker; 2, 8 h; 3, 24 h; 4, 48 h; 5, 56 h, and 6, 72 h. The membrane was probed with the anti-epimastigote whole extract antibody. Lanes 1 in (A), (B), (C), and (D) are the samples corresponding to control empty vector pBK-CMV HeLa transfected cells.Back to article page