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Figure 1 | Veterinary Research

Figure 1

From: Plasmid DNA immunization with Trypanosoma cruzi genes induces cardiac and clinical protection against Chagas disease in the canine model

Figure 1

rSSP4 and rSP protein expression in the HeLa plasmid transfected cells. (A) SDS-PAGE and (B) western blot analysis of the pBCSSP4 plasmid transfected cell extracts; samples were taken at different time points; Lanes: 2 and 3, 8 h; 4 and 5, 24 h; 6 and 7, 48 h; 8 and 9, 56 h. The membrane was probed with the anti-MBP::SSP4 antibody. (C) SDS-PAGE and (D) western blot analysis of the pBCSP plasmid transfected cell extracts; samples were taken at different time points; Lanes: MWM, molecular weight marker; 2, 8 h; 3, 24 h; 4, 48 h; 5, 56 h, and 6, 72 h. The membrane was probed with the anti-epimastigote whole extract antibody. Lanes 1 in (A), (B), (C), and (D) are the samples corresponding to control empty vector pBK-CMV HeLa transfected cells.

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