In vitro cytokine production by bone marrow-derived dendritic cells from naive C57BL/6 mice. Cells were stimulated with Neospora lysate antigen (NLA) at different concentrations (25, 10, 5 and 1 μg/mL), or Synadenium carinatum latex lectin (ScLL) at different concentrations (10, 1, 0.1 and 0.01 μg/mL) or NLA (25 μg/mL) plus ScLL (10, 1, 0.1 and 0.01 μg/mL). Cells stimulated with medium alone or LPS were included as controls. Cell-free supernatants were collected after 48 h and analyzed for detection of TNF-α (A), IL-6 (B), IL-10 (C) and IL-12 (D) by ELISA. Values are indicated as mean ± SD of cytokine levels in pg/mL. a-c Different letters indicate statistically significant differences between the concentrations in each group analyzed; *Statistically significant differences for the highest concentrations among groups (ANOVA and Bonferroni multiple comparison post-test; P < 0.05).