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Table 1 List of genes and sequences of the primers used for RT-PCR gene expression analysis

From: The local immune response of mice after Helicobacter suis infection: strain differences and distinction with Helicobacter pylori

Gene

Primer

Primer sequence

HPRT

sense

5- CAG GCC AGA CTT TGT TGG AT-3

antisense

5-TTG CGC TCA TCT TAG GCT TT-3

PPIA

sense

5-AGC ATA CAG GTC CTG GCA TC-3

antisense

5-TTC ACC TTC CCA AAG ACC AC-3

H2afz

sense

5-CGT ATC ACC CCT CGT CAC TT-3

antisense

5-TCA GCG ATT TGT GGA TGT GT-3

IL-1β

sense

5- GGG CCT CAA AGG AAA GAA TC-3

antisense

5-TAC CAG TTG GGG AAC TCT GC-3

IL-2

sense

5-TTT CAA TTG GAA GAT GCT GAG A-3

antisense

5-AGG GCT TGT TGA GAT GAT GC-3

IL-4

sense

5-ACT CTT TCG GGC TTT TCG AT-3

antisense

5-AAA AAT TCA TAA GTT AAA GCA TGG TG-3

IL-5

sense

5-GTG GGG GTA CTG TGG AAA TG-3

antisense

5-TCC TCG CCA CAC TTC TCT TT-3

IL-6

sense

5-CAA AGC CAG AGT CCT TCA GAG-3

antisense

5-GCC ACT CCT TCT GTG ACT CC-3

IFN-γ

sense

5- GCG TCA TTG AAT CAC ACC TG-3

antisense

5-TGA GCT CAT TGA ATG CTT GG-3

IL-10

sense

5-ATC GAT TTC TCC CCT GTG AA-3

antisense

5-CAC ACT GCA GGT GTT TTA GCT T-3

IL-12b

sense

5-TAA CCA GAA AGG TGC GTT CC-3’

antisense

5-CTT TCC AAC GTT GCA TCC TA-3

IL-17

sense

5-TTT AAC TCC CTT GGC GCA AAA-3

antisense

5-CTT TCC CTC CGC ATT GAC AC-3

TNF-α

sense

5-CAA ATG GCC TCC CTC TCA T-3

antisense

5-GGT TGT CTT TGA GAT CCA TGC-3

MIP-2

sense

5-AAA GTT TGC CTT GAC CCT GA-3

 

antisense

5-TCC AGG TCA GTT AGC CTT GC-3