Table 1 List of genes and sequences of the primers used for RT-PCR gene expression analysis
Gene | Primer | Primer sequence |
|---|---|---|
HPRT | sense | 5′- CAG GCC AGA CTT TGT TGG AT-3′ |
antisense | 5′-TTG CGC TCA TCT TAG GCT TT-3′ | |
PPIA | sense | 5′-AGC ATA CAG GTC CTG GCA TC-3′ |
antisense | 5′-TTC ACC TTC CCA AAG ACC AC-3′ | |
H2afz | sense | 5′-CGT ATC ACC CCT CGT CAC TT-3′ |
antisense | 5′-TCA GCG ATT TGT GGA TGT GT-3′ | |
IL-1β | sense | 5′- GGG CCT CAA AGG AAA GAA TC-3′ |
antisense | 5′-TAC CAG TTG GGG AAC TCT GC-3′ | |
IL-2 | sense | 5′-TTT CAA TTG GAA GAT GCT GAG A-3′ |
antisense | 5′-AGG GCT TGT TGA GAT GAT GC-3′ | |
IL-4 | sense | 5′-ACT CTT TCG GGC TTT TCG AT-3′ |
antisense | 5′-AAA AAT TCA TAA GTT AAA GCA TGG TG-3′ | |
IL-5 | sense | 5′-GTG GGG GTA CTG TGG AAA TG-3′ |
antisense | 5′-TCC TCG CCA CAC TTC TCT TT-3′ | |
IL-6 | sense | 5′-CAA AGC CAG AGT CCT TCA GAG-3′ |
antisense | 5′-GCC ACT CCT TCT GTG ACT CC-3′ | |
IFN-γ | sense | 5′- GCG TCA TTG AAT CAC ACC TG-3′ |
antisense | 5′-TGA GCT CAT TGA ATG CTT GG-3′ | |
IL-10 | sense | 5′-ATC GAT TTC TCC CCT GTG AA-3′ |
antisense | 5′-CAC ACT GCA GGT GTT TTA GCT T-3′ | |
IL-12b | sense | 5′-TAA CCA GAA AGG TGC GTT CC-3’ |
antisense | 5′-CTT TCC AAC GTT GCA TCC TA-3′ | |
IL-17 | sense | 5′-TTT AAC TCC CTT GGC GCA AAA-3′ |
antisense | 5′-CTT TCC CTC CGC ATT GAC AC-3′ | |
TNF-α | sense | 5′-CAA ATG GCC TCC CTC TCA T-3′ |
antisense | 5′-GGT TGT CTT TGA GAT CCA TGC-3′ | |
MIP-2 | sense | 5′-AAA GTT TGC CTT GAC CCT GA-3′ |
|  | antisense | 5′-TCC AGG TCA GTT AGC CTT GC-3′ |