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Table 3 An example of a scoring sheet used in a necrotic enteritis reproduction study

From: The successful experimental induction of necrotic enteritis in chickens by Clostridium perfringens: a critical review

Date and Time dd/mm/yy and am-pm

Cage # or Group # as appears on the cage

Number of birds in the cage / group

General behaviour*: Score

Comments

   

0

bright and alert;

 
   

1

reduced spontaneous activity

 
   

2

socially isolated but moves when approached

 
   

3

pronounced lethargy, only moves when stimulated

 
  1. *Actions to be taken.
  2. During experimental infection the birds will be observed every 4 h and the last observation will be made late evening around 8 pm. Birds will be examined around 2 AM, twice on day 3 and 4 of infection (approx. 12:30 AM, 4:30 AM).
  3. Birds showing scores of 1 will be head-marked with a marker pen. If the signs still persist 4 h later or if birds have or develop a score of 2 (or 3), such birds will be euthanized immediately using CO2.