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Table 1 Immunoreactive proteins of H. suis identified by LC-MS/MS

From: Immunization with the immunodominant Helicobacter suis urease subunit B induces partial protection against H. suis infection in a mouse model

Spot no.1 Protein name NCBI ID2 Gene pI3 MW3 No. matched peptides Mascot score4 Cov. (%)5
1 Urease subunit B EFX42254 ureB 5.97 62.967 117 1589 72
2 Urease subunit B EFX42254 ureB 5.97 62.967 80 1042 58
Threonyl-tRNA synthetase EFX41598 thrS 6.34 69.315 7 186 60
3 Urease subunit B EFX42254 ureB 5.97 62.967 80 903 46
4 Urease subunit B EFX42254 ureB 5.97 62.967 4 103 6
5 Urease subunit B EFX42254 ureB 5.97 62.967 4 103 6
6 30S ribosomal protein S1 EFX42427 rpsA 8.29 64.051 23 625 28
Quinone-reactive Ni/Fe hydrogenase, large subunit EFX41851 hydB 8.22 64.943 19 520 28
Urease of H. heilmannii AAA65722   8.86 25.844 8 258 26
7 Methyl-accepting chemotaxis protein EFX43528   7.1 48.907 35 905 50
8 Elongation factor G EFX41637 fusA 5.15 77.242 57 1066 55
9 Chaperonin GroEL EFX42237 groEL 5.58 58.498 150 3085 78
10 Chaperonin GroEL EFX42237 groEL 5.58 58.498 135 2647 73
Urease subunit B EFX42254 ureB 5.97 62.967 43 682 41
11 Flagellin A EFX41982 flaA 7.77 54.232 29 756 47
12 Flagellin A EFX41982 flaA 7.77 54.232 57 1294 62
13 Flagellin A EFX41982 flaA 7.77 54.232 46 1085 63
Trigger factor EFX42378 tig 5.13 49.587 12 343 24
14 Hydrogenase expression/formation protein EFX41790 hypB 5.59 27.617 15 314 35
Nicotinate-nucleotide pyrophosphorylase EFX42191 nadC 6.46 30.591 11 219 25
7-alpha-hydroxysteroid dehydrogenase EFX41880   6.62 28.246 6 186 24
Conserved hypothetical secreted protein EFX42511 hdhA 5.84 28.011 5 174 19
Hypothetical protein HSUHS5_0308 EFX42276   5.47 29.471 9 171 24
Peroxiredoxin EFX42277   5.84 25.811 7 107 19
15 Urease accessory protein EFX42255 ureH 6.79 30.447 4 106 13
16 Urease subunit A EFX42255 ureA 7.79 27.389 24 270 55
17 Urease subunit A EFX42255 ureA 7.79 27.389 28 112 45
18 Urease subunit A EFX42255 ureA 7.79 27.389 57 418 69
19 Urease subunit A EFX42255 ureA 7.79 27.389 75 568 73
  1. 1 Protein spot corresponding to position on gel and blots (see Figure 2).
  2. 2NCBI: National Center for Biotechnology Information.
  3. 3 Theoretical isoelectric point (pI) and molecular weight (MW).
  4. 4 For Helicobacter data, Mascot scores greater than 40 are significant (p ≤ 0.05).
  5. 5 % of the protein sequence covered by the peptides identified.