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Table 1 Immunoreactive proteins of H. suis identified by LC-MS/MS

From: Immunization with the immunodominant Helicobacter suis urease subunit B induces partial protection against H. suis infection in a mouse model

Spot no.1

Protein name

NCBI ID2

Gene

pI3

MW3

No. matched peptides

Mascot score4

Cov. (%)5

1

Urease subunit B

EFX42254

ureB

5.97

62.967

117

1589

72

2

Urease subunit B

EFX42254

ureB

5.97

62.967

80

1042

58

Threonyl-tRNA synthetase

EFX41598

thrS

6.34

69.315

7

186

60

3

Urease subunit B

EFX42254

ureB

5.97

62.967

80

903

46

4

Urease subunit B

EFX42254

ureB

5.97

62.967

4

103

6

5

Urease subunit B

EFX42254

ureB

5.97

62.967

4

103

6

6

30S ribosomal protein S1

EFX42427

rpsA

8.29

64.051

23

625

28

Quinone-reactive Ni/Fe hydrogenase, large subunit

EFX41851

hydB

8.22

64.943

19

520

28

Urease of H. heilmannii

AAA65722

 

8.86

25.844

8

258

26

7

Methyl-accepting chemotaxis protein

EFX43528

 

7.1

48.907

35

905

50

8

Elongation factor G

EFX41637

fusA

5.15

77.242

57

1066

55

9

Chaperonin GroEL

EFX42237

groEL

5.58

58.498

150

3085

78

10

Chaperonin GroEL

EFX42237

groEL

5.58

58.498

135

2647

73

Urease subunit B

EFX42254

ureB

5.97

62.967

43

682

41

11

Flagellin A

EFX41982

flaA

7.77

54.232

29

756

47

12

Flagellin A

EFX41982

flaA

7.77

54.232

57

1294

62

13

Flagellin A

EFX41982

flaA

7.77

54.232

46

1085

63

Trigger factor

EFX42378

tig

5.13

49.587

12

343

24

14

Hydrogenase expression/formation protein

EFX41790

hypB

5.59

27.617

15

314

35

Nicotinate-nucleotide pyrophosphorylase

EFX42191

nadC

6.46

30.591

11

219

25

7-alpha-hydroxysteroid dehydrogenase

EFX41880

 

6.62

28.246

6

186

24

Conserved hypothetical secreted protein

EFX42511

hdhA

5.84

28.011

5

174

19

Hypothetical protein HSUHS5_0308

EFX42276

 

5.47

29.471

9

171

24

Peroxiredoxin

EFX42277

 

5.84

25.811

7

107

19

15

Urease accessory protein

EFX42255

ureH

6.79

30.447

4

106

13

16

Urease subunit A

EFX42255

ureA

7.79

27.389

24

270

55

17

Urease subunit A

EFX42255

ureA

7.79

27.389

28

112

45

18

Urease subunit A

EFX42255

ureA

7.79

27.389

57

418

69

19

Urease subunit A

EFX42255

ureA

7.79

27.389

75

568

73

  1. 1 Protein spot corresponding to position on gel and blots (see Figure 2).
  2. 2NCBI: National Center for Biotechnology Information.
  3. 3 Theoretical isoelectric point (pI) and molecular weight (MW).
  4. 4 For Helicobacter data, Mascot scores greater than 40 are significant (p ≤ 0.05).
  5. 5 % of the protein sequence covered by the peptides identified.