Figure 2

H. suis 2D-proteome profile (A) and Western blots of a duplicate 2D-gel reacted with pooled sera of lysate-immunized mice (B) or of H. suis -infected mice (C). 100 μg of total protein extract of H. suis was separated by 2D-electrophoresis using linear pH3 to10 gradient in the first dimension and 10% TrisHCl SDS-PAGE in the second dimension. The separated proteins were detected by SYPRO®Ruby Protein staining. The boxed areas indicate where immunoreactive antigens were excised from the gel and subjected to LC-MS/MS. Identified proteins are indicated by the spot numbers given in Table 1. Boxes and numbers in red were identified as UreB. The position of molecular weight (MW) is given on the right, and the pH is given at the bottom.