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Table 1 PCR primer pairs and reaction conditions used in the work described in this paper

From: Ganjam virus/Nairobi sheep disease virus induces a pro-inflammatory response in infected sheep

Target

Primer sequences

Ta1

[Primer]2

Reference

NSDV/GV (F1/R1A)

TGACCATGCAGAACCAGATYG

62

300nM

this paper

GAAACAAGCCTCATGCTAACCT

   

NSDV/GV (F2/R2)

GGAGAATGGCAAAGAGGTTGT

64

300nM

this paper

GTAAATCCGATTGGCAGTGAAG

   

NSDV/GV F3b (RT primer)

GTCTTTGAACTYTGACCA

n/a

n/a

this paper

IL-1β

CCTTGGGTATCAGGGACAA

60

300nM

[16]

TGCGTATGGCTTTCTTTAGG

   

IL-4

ACCTGTTCTGTGAATGAAGCCAA

60

300nM

[17]

CCCTCATAATAGTCTTTAGCCTTTCC

   

IL-6

TCCAGAACGAGTTTGAGG

60

400nM

[16]

CATCCGAATAGCTCTCAG

   

IL-8

ATGAGTACAGAACTTCGA

57

300nM

[16]

TCATGGATCTTGCTTCTC

   

IL-10

TGCTGTTGACCCAGTCTCTG

60

200nM

this paper

AGGGCAGAAAACGATGACAG

   

IL-12A

TGGGCATTGTCTGTCTTCTG

60

200nM

this paper

TTCTTCCAGGGAGGGTTTCT

   

IL-12B

GCTGGGAGTACCCTGACACG

61

500nM

[17]

GTGACTTTGGCTGAGGTTTGGTC

   

IL-18

ACTGTTCAGATAATGCACCCCAG

60

300nM

[17]

TTCTTACACTGCACAGAGATGGTTAC

   

Interferon β

CCAGATGGTTCTCCTGCTGTGT

63

300nM

this paper

GACCAATACGGCATCTTCCTTC

   

TNFα

GAATACCTGGACTATGCCGA

60

200nM

[16]

CCTCACTTCCCTACATCCCT

   

TGFβ

GTGGACATCAACGGGTTCAG

60

300nM

this paper

TGTCCAGGCTCCAGATGTAG

   

Interferon γ

CTCCGGCCTAACTCTCTCCT

60

300nM

this paper

AGGCCCACCCTTAGCTACAT

   

GAPDH

GGTGATGCTGGTGCTGAGTA

60

300nM

[16]

TCATAAGTCCCTCCACGATG

   

SDHA

ACCTGATGCTTTGTGCTCTGC

60

200nM

[16]

CCTGGACGGGCTTGGAGTAA

   

G6PDH

CGAGGCTGTGTACACCAAGA

60

300nM

this paper

 

ATGTGGTGGAGCAGTGGAGT

   
  1. 1: Ta: annealing temperature.
  2. 2: [Primer]: primer concentration.