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Table 1 Efficacy of the immunisation with live Nc-Spain 1 H tachyzoites against congenital and cerebral infection in mice

From: Evaluation of the protection conferred by a naturally attenuated Neospora caninum isolate against congenital and cerebral neosporosis in mice

  Congenital infection Cerebral infectionh
Group Litter sizea Stillbirth Neonatal mortality Vertical transmission
   Per pupsb Per littersc Per pupsd Per litterse Per pupsf Per littersg  
5 × 10 5 /challenged 5.0 ± 2.2 4/45 (8.8%) 2/9 (22.2%) 1/41 (2.4%) 1/8 (12.5%) 1/44 (2.3%) 1/9 (11.1%) 0/16 (0%)
Non-immunised/challenged 5.2 ± 1.7 8/58 (13.8%) 5/11 (45.5%) 42/50 (84%) 11/11 (100%) 41/46 (89.1%) 11/11 (100%) 13/16 (81.25%)
Non-immunised/non-challenged 4.6 ± 1.4 0/37 (0%) 0/8 (0%) 0/37 (0%) 0/8 (0%) 0/37 (0%) 0/8 (0%) 0/17 (0%)
5 × 10 5 /challenged 4.6 ± 2.0 5/41 (12.2%) 4/9 (44.4%) 6/36 (16.6%) 2/9 (22.2%) 5/34 (14.7%) 4/9 (44.4%) 2/19 (10.5%)
5 × 10 4 /challenged 5.3 ± 1.3 8/79 (10.1%) 5/15 (33.3%) 8/71 (11.3%) 6/15 (40%) 9/75 (12%) 6/15 (40%) 1/24 (4.16%)
5 × 10 3 /challenged 5.8 ± 1.4 28/93 (30.1%) 11/16 (68.8%) 17/65 (26.1%) 7/15 (46.6%) 26/86 (30.2%) 7/15 (47%) 0/24 (0%)
5 × 10 2 /challenged 4.1 ± 1.5 4/41 (9.7%) 3/10 (30%) 7/37 (18.9%) 6/10 (60%) 14/41 (34.1%) 5/10 (50%) 1/26 (3.8%)
5 × 10/challenged 4.4 ± 1.9 14/44 (31.8%) 5/10 (50%) 2/30 (6.6%) 2/9 (22.2%) 2/37 (5.4%) 2/9 (22.2%) 2/25 (8%)
Non-immunised/challenged 5.0 ± 1.7 25/70 (35.7%) 8/14 (57.1%) 38/45 (84.4%) 13/13 (100%) 37/51 (72.6%) 13/13 (100%) 17/24 (70.8%)
Non-immunised/non-challenged 5.3 ± 1.6 3/64 (4.7%) 3/12 (25%) 0/61 (0%) 0/12 (0%) 0/64 (0%) 0/12 (0%) 0/24 (0%)
  1. Experiment no. 1. Mice were randomly allocated in groups of 21–22 mice in experiment no. 1.
  2. Experiment no. 2. The number of mice per group in experiment no. 2 was expanded to 29–31 mice to improve the statistical power by increasing the number. of pregnant mice per group.
  3. a Average ± SD.
  4. b No. of stillborns/no. of total pups born in the group (percentage).
  5. c No. of litters with at least one stillborn/no. of litters in the group (percentage).
  6. d No. of pups dead from birth to 30 days PP/no. of pups born alive (percentage).
  7. e No. of litters with at least one pup dead from birth to 30 days PP/no. of litters in the group (percentage).
  8. f No. of nested PCR-positive pups/no. of analysed pups (percentage).
  9. g No. of litters with at least one nested PCR-positive pup/no. of analysed litters (percentage).
  10. h No. of nested PCR-positive adult mice/no. of analysed adult mice in the group at the chronic infection phase (percentage).