Figure 4
Panel A. ELISA using anti- N.caninum IgG1 and IgG2a from the BALB/c mice that were immunised with various doses (5 × 105-5 × 10) of Nc-Spain 1 H at day 5 after booster (panel A). The bars represent the optical density (OD) at 405 nm, and the error bars indicate the SEM for each group. Positive cut-offs were established in ELISA for IgG1 detection at ≥ 0.132 and in ELISA for IgG2a detection at ≥ 0.131. A total of 5 mice prior to challenge were included in the analysis. Panel B. Bars represent the average of anti-N. caninum IgG1:IgG2a isotype ratios from the BALB/c mice that were immunised with various doses (5 × 105-5 × 10) of Nc-Spain 1 H at day 5 after booster. Error bars represent the ± SEM. The discontinuous line marks identical IgG1 and IgG2a levels (IgG1:IgG2a = 1).