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Figure 6 | Veterinary Research

Figure 6

From: Stimulation with a class A CpG oligonucleotide enhances resistance to infection with feline viruses from five different families

Figure 6

Supernatants derived from ODN 2216-stimulated PBMCs decrease retroviral DNA and RNA loads in target cells. (A) FEA cells were incubated for 24 h with the respective supernatants or medium alone, before inoculation with the feline leukaemia virus (FeLV), as well as every 2 days thereafter. Viral RNA loads were measured at the indicated time points by real time RT-PCR and 45-Ct values are depicted. (B) FeLV DNA loads in the cells were measured at the indicated time points and Ct values were normalized to detection of a housekeeping gene (GAPDH). Mean values from duplicate experiments carried out simultaneously with the supernatants derived from PBMCs of a selected cat (belonging to group 2) and with medium alone are shown as an example. Results for (A) and (B) are indicative of those obtained with supernatants from PBMCs of two additional adult cats (from group 3). Stars represent statistical differences in area under the curve (AUC) measurements between the curves of all three cats obtained in cells incubated with Sup 2216 and each of the other treatments. Mx mRNA expression factors (C), viral loads (45-Ct values depicted) (D) and proviral loads (E) were measured in the FEA cells of five cats (two from group 2 and three from group 3) on day 8 post inoculation. Each dot represents the mean of duplicate measurements for an individual cat. *p < 0.05, **p < 0.01.

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