Figure 5

Supernatants derived from ODN 2216 stimulated PBMCs inhibit viral replication in target cells. (A-E) fcwf-4 cells were incubated for 24 h with the indicated supernatants derived from PBMCs of eight adult cats (groups 2 and 3) or medium only as control before inoculation with the indicated viruses. Each dot represents mean optical density (OD) values from spectrophotometric readings of viral inhibition assays conducted on duplicate wells treated with supernatants from an individual cat. (F-J) Correlation of individual inhibition ratios of each virus with Mx mRNA expression induced in fcwf-4 cells incubated with supernatants of ODN 2216 stimulated PBMCs from the eight cats of groups 2 and 3. Note the different scale on the x-axis for each graph indicating the differences in the inhibitory effects of these supernatants on the different viruses. (K, L) fcwf-4 cells were incubated for 24 h with the indicated supernatants derived from PBMCs of 4 kittens (group 1) or medium only as control before inoculation with VSV or FCV. Each dot represents mean OD values from spectrophotometric readings of viral inhibition assays conducted on duplicate wells treated with supernatants from one cat. **p < 0.01. VSV = vesicular stomatitis virus, FCV = feline calicivirus, FPV = feline parvovirus, FCoV = feline coronavirus, FHV = feline herpes virus.