Figure 3

ODN 2216 influence IFNα and IL-6 expression in primary feline immune cells. mRNA expression factors of IFNα and IL-6 were measured in (A, B) fcwf-4 cells stimulated with ODN 2216 and (C-F) feline PBMCs from one cat belonging to group 2 stimulated with either ODN 2216 or control ODN 2243. The transcription of both genes was assessed either 24 h after treatment of the cells with increasing concentrations of ODN (A, C, D), or over time after a single stimulation with 4 μg/mL ODN (B, E, F). Experiments C-F were repeated for 4 adult cats and representative results are shown.