Skip to main content
Figure 1 | Veterinary Research

Figure 1

From: Stimulation with a class A CpG oligonucleotide enhances resistance to infection with feline viruses from five different families

Figure 1

ODN 2216 induce proliferation of primary feline immune cells and enhance their expression of costimulatory surface molecules. (A) The proliferation of feline PBMCs of eight adult cats belonging to groups 2 and 3 after stimulation with ODN 2216 and ODN 2243 was assessed by H3-thymidine incorporation. The dots indicate the stimulation index calculated from triplicate cultures for each cat. (B and C) Expression levels of B7.1 and MHCII surface molecules were assessed 24 h post stimulation with the indicated treatments by flow cytometry. The fluorescence of gated PBMCs of one cat selected as an example is depicted. Isotype control samples are indicated as unstained. (D-G) Mean fluorescence intensity (MFI) for B7.1 (D and E) and MHCII (F and G) of gated lymphocytes and non-lymphocyte subpopulations stimulated with the indicated treatments. Results for eight cats belonging to groups 2 and 3 are shown. *p < 0.05, **p < 0.01.

Back to article page