Progression of CyHV-3 infection in carp analysed by bioluminescence imaging. Two groups of fish (mean weight of 10 g) were infected with the CyHV-3 LUC strain either by bathing them in water containing the virus (Immersion, left column) or by feeding them with food pellets contaminated with the virus (Oral, right column). At the indicated time post-infection, six fish per group were analysed by in vivo and ex vivo bioluminescence imaging. A/For each fish, the IVIS signal (Photon/second) was determined for several organs (skin, gills, pharynx, pseudogaster, intestine, and heart) as described in the materials and methods. B/For each analysed organ, the number of positive fish is presented according to time post-infection. This experiment is representative of two independent experiments.