Figure 6
From: VtaA8 and VtaA9 from Haemophilus parasuis delay phagocytosis by alveolar macrophages
Intracellular localization of the E. coli clones carrying vtaA8 and vtaA9 from H. parasuis within the endosomal network of PAM at different times post-infection (30 min and 1 h). E. coli (pACYC184 pEGFP) (Control, first column) and the clones with vtaA8 (pMCH-vtaA8 pEGFP; vtaA8, second column) or vtaA9 (pMCH-vtaA9 pEGFP; vtaA9, third column) were expressing GFP (green signal). Nuclei were counterstained with DAPI (blue signal). Upper panels show the co-localization (arrows) between control, vtaA8 or vtaA9 and the early endosomal marker EEA1 (red signal). Lower panels show the co-localization (arrows) between the control, vtaA8 or vtaA9 and the acidic lysosomes stained with LysoTracker Red DND-99 (red signal). Percentages of co-localization are indicated in each panel. Scale bars, 5 μm. Individual images for each fluorescence are presented in the Additional file 2: Figure S2A and Figure S2B.