Molecular characteristics of PrPres(WB with P4 antibody) in obex samples. Sheep recipients of the different transmission groups are colour-coded to match the IHC profiles shown in Figure 3. A: Molecular weights (MWs in kDa) of the unglycosylated band: note the association between AAS inoculum (circles), A and A’ PrPd profiles (red and pink, respectively) and generally lower MWs, and between VVC inoculum (squares), M and M’ IHC profiles (green and light blue, respectively) and generally higher MWs (for statistical analysis refer to text). B: Triplot representation of the glycoprofiles as proportion of unglycosylated (ung., green scale and grid), monoglycosylated (monog, black scale and grid) and diglycosylated band (dig, blue scale and grid): note that 11 of 12 sheep with M or M’ IHC profiles have more than 26% of unglycosylated PrPres, whereas 18 of 23 with A or A’ PrPd profiles have 26% or less unglycosylated PrPres (dotted green line). Similarly, 11 of 12 sheep with M or M’ IHC profiles have less than 43% of diglycosylated PrPres, whereas 15 of 23 with A or A’ PrPd profiles have 43% or more diglycosylated PrPres (dotted blue line). For statistical analyses refer to text. The only one sheep with a CH type of PrPd profile (see Figure 3) is not represented as it did not react with P4 antibody in the WB.