Figure 4

Examples of the brain PrP^dprofile types encountered in different transmission groups. A: recipient AAS1: type A profile in cerebral cortex (note similarities with Figure 2a); inset, recipient AAS10 (type A) illustrating the absence of intracellular PrP^d in deep cerebellar nuclei despite the presence of abundant extracellular aggregates. B: recipient AAS17: type A’ profile in corpus striatum: peri-vascular PrP^d in capsula interna and abundant extracellular (peri-neuronal, linear and particulate) PrP^d in neuropil but inconspicuous intraneuronal PrP^d in deep cerebellar nuclei (inset, same recipient). C: recipient VVC11 (type M’) showing co-occurrence of extracellular (linear, perineurinal, particulate and coalescing) and intraneuronal PrP^d in corpus striatum; inset: recipient VVC12 showing the same co-occurrence of intra- and extra-cellular PrP^d in deep cerebellar nuclei (type M'). D: recipient VVC1: type M profile with almost complete absence of extracellular glia-associated PrP^d and some neuropil-associated and intracellular aggregates in deep cerebral cortex layers; inset, same recipient showing abundant intraneuronal PrP^d in deep cerebellar nuclei. E: recipient VVC13 (type P profile) showing abundant extracellular PrP^d in the neuropil of the thalamus and prominent non-vascular, Kuru-type plaques (detail in inset). F: recipient VVC14 (type CH profile) showing conspicuous intracellular PrP^d deposits in Purkinje cells and in neurons and glial cells within the red nucleus (inset). IHC with R145 PrP monoclonal antibody and haematoxylin counterstaining. Original magnifications: large images × 4 (except F, ×10); insets × 60.