Figure 5

The total amount of MHC II molecules in porcine macrophages is decreased 24 h post inoculation with Salmonella Typhimurium strain 112910a. Macrophages were either or not inoculated with Salmonella Typhimurium strain 112910a at MOI 10. Whole cell lysates were prepared at 0 and 24 h post inoculation. Eight μL of each lysate were spotted on a nitrocellulose membrane and air dried. The membrane was incubated with a primary mouse anti-pig SLA class II DQ antibody, and subsequently with a secondary HRP-conjugated goat anti-mouse IgG antibody. The CN/DAB substrate kit was applied onto the membrane and the membrane was scanned with a GS-800 densitometer