Figure 2From: Rescue of recombinant peste des petits ruminants virus: creation of a GFP-expressing virus and application in rapid virus neutralization testReplication of and GFP expression by rPPRV/GFP in Vero cells. (A) Cells infected with either rPPRV/GFP (i, iv, vii) or PPRV/N75/1 (ii, v, viii), or mock infected (iii, vi, ix), were fixed and labeled for the presence of PPRV glycoprotein H (red); GFP was detected by its natural fluorescence. Cells were counterstained with DAPI to show the nuclei. (B) GFP expression in live, PPRV/N75/1- and rPPRV/GFP-infected Vero cells was observed by direct observation of unfixed cells 2 days post infection. (C) Lysates of Vero cells that had been infected with PPRV/N75/1 or rPPRV/GFP were analyzed by Western blotting using mouse anti-GFP monoclonal antibody. Mock-infected Vero cells were used as a controls.Back to article page