Figure 1

Bovine Tim-3. (A) Alignment of deduced amino acid sequences of Tim-3. Dots indicate identity to the sequence from Holstein breeds and dashes indicate gaps in alignment. Two potential N-glycosylation sites are shown in bold and underlined. The potential tyrosine kinase phosphorylation motif is indicated in the box. The accession numbers of analyzed sequences corresponded to those in Table 1. (B) A phylogenetic tree constructed based on the amino acid sequences of Tim-3. The tree was built with the neighbor-joining method using the MEGA 5.0 software. Numbers indicate bootstrap percentage (1000 replicates). The scale indicates the divergence time.