|
Groupa
|
Challengeb
|
|---|
|
Gross lesiond
|
Bacterial recovery
|
|---|
|
Mortality (%)
|
Liver
|
Spleen
|
Liver
|
Spleen
|
|---|
|
Positive No.
|
Positive No.
|
|---|
|
A
|
11/15 (73)c
|
2. 2.16 ± 1.46e**
|
1.70 ± 1.46**
|
0/5f
|
0/5
|
|
B
|
2/15 (14)
|
1.06 ± 0.96
|
0.66 ± 1.12
|
0/5
|
0/5
|
|
C
|
1/15 (6.6)
|
0.60 ± 0.98
|
0.53 ± 0.83
|
0/5
|
0/5
|
|
D
|
1/15 (6.6)
|
0.53 ± 0.89
|
0.53 ± 0.93
|
0/5
|
0/5
|
- a Immunization was performed at day 7 of age with the Salmonella Gallinarum ghost strain and the groups were designated as group A (non vaccinated), group B (orally immunized), group C (immunized subcutaneously) and group D (immunized intramuscularly). Fifteen birds were allocated per group.
- b Challenge was performed with a wild type Salmonella Gallinarum strain using 1 × 106 CFU after 21 days post vaccination.
- c Number of dead birds upon challenge.
- d Gross lesion was observed at day14 post challenge.
- e Group lesion score (Mean ± SD).
- f Number of positive samples after enrichment culture. All values were considered to be significant if p ≤ 0.05 or 0.01.* p < 0.05, **p < 0.01 vs. non vaccinated group A.
