Groupa | Challengeb |
|---|
Gross lesiond | Bacterial recovery |
|---|
Mortality (%) | Liver | Spleen | Liver | Spleen |
|---|
Positive No. | Positive No. |
|---|
A | 11/15 (73)c | 2. 2.16 ± 1.46e** | 1.70 ± 1.46** | 0/5f | 0/5 |
B | 2/15 (14) | 1.06 ± 0.96 | 0.66 ± 1.12 | 0/5 | 0/5 |
C | 1/15 (6.6) | 0.60 ± 0.98 | 0.53 ± 0.83 | 0/5 | 0/5 |
D | 1/15 (6.6) | 0.53 ± 0.89 | 0.53 ± 0.93 | 0/5 | 0/5 |
- a Immunization was performed at day 7 of age with the Salmonella Gallinarum ghost strain and the groups were designated as group A (non vaccinated), group B (orally immunized), group C (immunized subcutaneously) and group D (immunized intramuscularly). Fifteen birds were allocated per group.
- b Challenge was performed with a wild type Salmonella Gallinarum strain using 1 × 106 CFU after 21 days post vaccination.
- c Number of dead birds upon challenge.
- d Gross lesion was observed at day14 post challenge.
- e Group lesion score (Mean ± SD).
- f Number of positive samples after enrichment culture. All values were considered to be significant if p ≤ 0.05 or 0.01.* p < 0.05, **p < 0.01 vs. non vaccinated group A.
