Flow cytometric analysis for CD3+CD4+and CD3+CD8+T-lymphocyte populations. A) Flow cytometry scatter dot plots for CD3+, CD4+, CD8+ T-cell populations. The plots represent events for one representative chicken from each group. The gating and the quadrants are set according to the standard procedures of the BD-Biosciences flow cytometer B) Bar-graphs represent CD3+ CD4+ T-lymphocyte populations in immunized and non-immunized chickens C) Bar-graphs represent CD3+ CD8+ T-lymphocyte populationin immunized and non immunized chickens. Values are shown as Mean ± S.D of 5 chickens per group and were considered statistically significant, if p values were ≤ 0.05 (*p ≤ 0.05). Group A, non- immunized control; group B, orally immunized; group C, immunized subcutaneously; group D, intramuscularly immunized.