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Table 2 Multilocus genotyping of Toxoplasma gondii clones isolated from in-vitro cell cultures of T. gondii by limiting dilution

From: Toxoplasma gondii sexual cross in a single naturally infected feline host: Generation of highly mouse-virulent and avirulent clones, genotypically different from clonal types I, II and III

Designation

Origin (number of clones)

PCR-RFLP genotype - genetic marker (location*)

nSAG2 (Chr.VIII)

SAG3 (Chr.XII)

BTUB (Chr.IX)

GRA6 (Chr.X)

c22-8 (Chr.Ib)

c29-2 (Chr.III)

L358 (Chr.V)

PK1 (Chr.VI)

Apico (Plastid)

Group 1 (16 clones)

K119/2 a (10), B136/4 b (4),K119/1 (1), B136/5 (1)

III

III

III

III

III

III

III

III

III

Group 2 (22 clones)

B136/2 (13), K119/1 (4), K119/2 (4), B136/4 (1)

III

III

III

III

III

III

III

II

III

Group 3 (2 clones)

K119/2 (2)

III

III

II

III

III

II

III

III

III

Group 4 (4 clones)

B136/1 (3), B136/5 (1)

II

III

III

III

II

III

II

II

III

Group 5 (1 clone)

B136/4 (1)

II

III

III

III

II

III

II

II

I

  1. Typing was performed by PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. T. gondii clones were grouped according to their allele pattern.
  2. a K, T. gondii cell culture clone obtained from infected GKO mouse.
  3. b B, T. gondii cell culture clone obtained from infected BALB/c mouse.
  4. * Chr., chromosome.
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Veterinary Research

ISSN: 1297-9716