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Table 2 Multilocus genotyping of Toxoplasma gondii clones isolated from in-vitro cell cultures of T. gondii by limiting dilution

From: Toxoplasma gondii sexual cross in a single naturally infected feline host: Generation of highly mouse-virulent and avirulent clones, genotypically different from clonal types I, II and III

Designation Origin (number of clones) PCR-RFLP genotype - genetic marker (location*)
nSAG2 (Chr.VIII) SAG3 (Chr.XII) BTUB (Chr.IX) GRA6 (Chr.X) c22-8 (Chr.Ib) c29-2 (Chr.III) L358 (Chr.V) PK1 (Chr.VI) Apico (Plastid)
Group 1 (16 clones) K119/2 a (10), B136/4 b (4),K119/1 (1), B136/5 (1) III III III III III III III III III
Group 2 (22 clones) B136/2 (13), K119/1 (4), K119/2 (4), B136/4 (1) III III III III III III III II III
Group 3 (2 clones) K119/2 (2) III III II III III II III III III
Group 4 (4 clones) B136/1 (3), B136/5 (1) II III III III II III II II III
Group 5 (1 clone) B136/4 (1) II III III III II III II II I
  1. Typing was performed by PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. T. gondii clones were grouped according to their allele pattern.
  2. a K, T. gondii cell culture clone obtained from infected GKO mouse.
  3. b B, T. gondii cell culture clone obtained from infected BALB/c mouse.
  4. * Chr., chromosome.