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Table 1 Multilocus genotyping of Toxoplasma gondii isolates and clones originating from a single oocyst sample by PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis

From: Toxoplasma gondii sexual cross in a single naturally infected feline host: Generation of highly mouse-virulent and avirulent clones, genotypically different from clonal types I, II and III

Designation

Inoculum

PCR-RFLP genotype - genetic marker (location*)

nSAG2 (Chr.VIII)

SAG3 (Chr.XII)

BTUB (Chr.IX)

GRA6 (Chr.X)

c22-8 (Chr.Ib)

c29-2 (Chr.III)

L358 (Chr.V)

PK1 (Chr.VI)

Apico (Plastid)

RH

 

I

I

I

I

I

I

I

I

I

Me49

 

II

II

II

II

II

II

II

II

II

NED

 

III

III

III

III

III

III

III

III

III

TG-GER63a

 

I or III + II

II

II

III

II + III

II + III

III

II + III

III

K119/1b

Oocysts (TG-GER63)

III

II

III

II + III

II + III

II + III

II + III

II

III

K119/2

Oocysts (TG-GER63)

III

II

III

III

II + III

III

III

III

III

B136/1c

Tachyzoites (K119/1)

II + III

III

III

II + III

II + III

III

II + III

II + III

III

B136/2

Tachyzoites (K119/1)

II + III

III

III

II + III

III

III

II + III

II + III

III

B136/3

Tachyzoites (K119/1)

II + III

III

III

II + III

III

III

II + III

II + III

III

B136/4

Tachyzoites (K119/1)

II + III

III

II + III

II + III

III

III

II + III

II + III

III

B136/5

Tachyzoites (K119/1)

II + III

III

III

II + III

II + III

III

II + III

II + III

III

  1. Two interferon-γ-knockout (GKO) mice (K119/1 and K119/2) were orally inoculated with sporulated oocysts of TG-GER63a. Tachyzoites isolated from the peritoneal fluid and brain tissue of K119/1 were used to infect five BALB/c mice (B136/1-5) intra-peritoneally and to establish in-vitro tissue cultures of these parasites in VERO cells. Three reference strains including RH, Me49 and NED were included in each PCR-RFLP run.
  2. a as described by [15].
  3. b K, T. gondii cell-culture clone obtained from infected GKO mouse.
  4. c B, T. gondii cell-culture clone obtained from infected BALB/c mouse.
  5. * Chr., chromosome.
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Veterinary Research

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