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Table 1 Multilocus genotyping of Toxoplasma gondii isolates and clones originating from a single oocyst sample by PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis

From: Toxoplasma gondii sexual cross in a single naturally infected feline host: Generation of highly mouse-virulent and avirulent clones, genotypically different from clonal types I, II and III

Designation Inoculum PCR-RFLP genotype - genetic marker (location*)
nSAG2 (Chr.VIII) SAG3 (Chr.XII) BTUB (Chr.IX) GRA6 (Chr.X) c22-8 (Chr.Ib) c29-2 (Chr.III) L358 (Chr.V) PK1 (Chr.VI) Apico (Plastid)
RH   I I I I I I I I I
Me49   II II II II II II II II II
NED   III III III III III III III III III
TG-GER63a   I or III + II II II III II + III II + III III II + III III
K119/1b Oocysts (TG-GER63) III II III II + III II + III II + III II + III II III
K119/2 Oocysts (TG-GER63) III II III III II + III III III III III
B136/1c Tachyzoites (K119/1) II + III III III II + III II + III III II + III II + III III
B136/2 Tachyzoites (K119/1) II + III III III II + III III III II + III II + III III
B136/3 Tachyzoites (K119/1) II + III III III II + III III III II + III II + III III
B136/4 Tachyzoites (K119/1) II + III III II + III II + III III III II + III II + III III
B136/5 Tachyzoites (K119/1) II + III III III II + III II + III III II + III II + III III
  1. Two interferon-γ-knockout (GKO) mice (K119/1 and K119/2) were orally inoculated with sporulated oocysts of TG-GER63a. Tachyzoites isolated from the peritoneal fluid and brain tissue of K119/1 were used to infect five BALB/c mice (B136/1-5) intra-peritoneally and to establish in-vitro tissue cultures of these parasites in VERO cells. Three reference strains including RH, Me49 and NED were included in each PCR-RFLP run.
  2. a as described by [15].
  3. b K, T. gondii cell-culture clone obtained from infected GKO mouse.
  4. c B, T. gondii cell-culture clone obtained from infected BALB/c mouse.
  5. * Chr., chromosome.