Designation
|
Inoculum
|
PCR-RFLP genotype - genetic marker (location*)
|
---|
nSAG2 (Chr.VIII)
|
SAG3 (Chr.XII)
|
BTUB (Chr.IX)
|
GRA6 (Chr.X)
|
c22-8 (Chr.Ib)
|
c29-2 (Chr.III)
|
L358 (Chr.V)
|
PK1 (Chr.VI)
|
Apico (Plastid)
|
---|
RH
| |
I
|
I
|
I
|
I
|
I
|
I
|
I
|
I
|
I
|
Me49
| |
II
|
II
|
II
|
II
|
II
|
II
|
II
|
II
|
II
|
NED
| |
III
|
III
|
III
|
III
|
III
|
III
|
III
|
III
|
III
|
TG-GER63a
| |
I or III + II
|
II
|
II
|
III
|
II + III
|
II + III
|
III
|
II + III
|
III
|
K119/1b
|
Oocysts (TG-GER63)
|
III
|
II
|
III
|
II + III
|
II + III
|
II + III
|
II + III
|
II
|
III
|
K119/2
|
Oocysts (TG-GER63)
|
III
|
II
|
III
|
III
|
II + III
|
III
|
III
|
III
|
III
|
B136/1c
|
Tachyzoites (K119/1)
|
II + III
|
III
|
III
|
II + III
|
II + III
|
III
|
II + III
|
II + III
|
III
|
B136/2
|
Tachyzoites (K119/1)
|
II + III
|
III
|
III
|
II + III
|
III
|
III
|
II + III
|
II + III
|
III
|
B136/3
|
Tachyzoites (K119/1)
|
II + III
|
III
|
III
|
II + III
|
III
|
III
|
II + III
|
II + III
|
III
|
B136/4
|
Tachyzoites (K119/1)
|
II + III
|
III
|
II + III
|
II + III
|
III
|
III
|
II + III
|
II + III
|
III
|
B136/5
|
Tachyzoites (K119/1)
|
II + III
|
III
|
III
|
II + III
|
II + III
|
III
|
II + III
|
II + III
|
III
|
- Two interferon-γ-knockout (GKO) mice (K119/1 and K119/2) were orally inoculated with sporulated oocysts of TG-GER63a. Tachyzoites isolated from the peritoneal fluid and brain tissue of K119/1 were used to infect five BALB/c mice (B136/1-5) intra-peritoneally and to establish in-vitro tissue cultures of these parasites in VERO cells. Three reference strains including RH, Me49 and NED were included in each PCR-RFLP run.
- a as described by [15].
- b K, T. gondii cell-culture clone obtained from infected GKO mouse.
- c B, T. gondii cell-culture clone obtained from infected BALB/c mouse.
- * Chr., chromosome.