Figure 7

Akt phosphorylation at early phase of infection induced by UV-inactivated influenza A viruses. MDCK cells grown in serum-free medium were infected with UV-inactivated influenza A viruses (MOI = 2) for indicated times. Cellular lysates were used for Western blotting using specific antibodies for phospho-Akt(Ser473), total-Akt, β-actin, NP, or NS1. Note that no NP proteins were detected except for unspecific bands. Quantity one software was applied to analyze the signal intensities of phospho-Akt and total-Akt and the relative ratios were presented