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Figure 6 | Veterinary Research

Figure 6

From: Inability of NS1 protein from an H5N1 influenza virus to activate PI3K/Akt signaling pathway correlates to the enhanced virus replication upon PI3K inhibition

Figure 6

Variation of Akt phosphorylation during influenza A virus infection. Serum- starved MDCK cells were infected with different influenza A viruses at an MOI of 2. Cells were lysed at indicated postinfection time points and subjected to Western blotting using specific antibodies for phospho-Akt(Ser473), total-Akt, β-actin, NP, or NS1. Signal intensities of phospho-Akt and total-Akt were quantified by Quantity one software and the ratios were shown at the bottom.

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