Figure 6

Variation of Akt phosphorylation during influenza A virus infection. Serum- starved MDCK cells were infected with different influenza A viruses at an MOI of 2. Cells were lysed at indicated postinfection time points and subjected to Western blotting using specific antibodies for phospho-Akt(Ser473), total-Akt, β-actin, NP, or NS1. Signal intensities of phospho-Akt and total-Akt were quantified by Quantity one software and the ratios were shown at the bottom.