Figure 5

In vivo association of NS51 with p85β. Hela cells were transfected for 36 h with either PNF-NS51 plasmid or PNF empty vector. Soluble cellular lysates were immunoprecipitated with rabbit anti-Flag antibody or normal rabbit IgG. Precipitated proteins were separated by SDS-PAGE, followed by Western blot analysis using mouse anti-NS1 antibody or mouse anti-p85β antibody and Protein G-HRP.