Figure 5From: Inability of NS1 protein from an H5N1 influenza virus to activate PI3K/Akt signaling pathway correlates to the enhanced virus replication upon PI3K inhibitionIn vivo association of NS51 with p85β. Hela cells were transfected for 36 h with either PNF-NS51 plasmid or PNF empty vector. Soluble cellular lysates were immunoprecipitated with rabbit anti-Flag antibody or normal rabbit IgG. Precipitated proteins were separated by SDS-PAGE, followed by Western blot analysis using mouse anti-NS1 antibody or mouse anti-p85β antibody and Protein G-HRP.Back to article page