Analysis of the interactions between NS1 proteins and p85β using yeast trap assays. A) AH109 yeasts transformed with the indicated plasmids were firstly plated onto DDO plates for 4 days of incubation at 30°C. And then, single AH109 colonies were picked and streaked on QDO/X-α-gal agar plate and incubated at 30°C for 3 days. The representative colonies were presented. (B) Different NS1-encoding plasmids were transformed into AH109 yeasts together with pGBK-p85β plasmid for α-galactosidase activities assays in liquid culture using PNP-α-gal as substrate. Data were from three independent experiments. BK-p53 + AD-T and BK-Lam + AD-T represented positive and negative control, respectively. Statistical comparison of different values was performed by the Student t test. Groups marked with asterisks are statistically different from the negative group (P < 0.01).