The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig

Table 2 Relative mRNA expression of TLR (−2, -4, -2/6 and −7) after T-2 toxin treatment in PAM

Primary porcine cells (2 × 10⁶ cells per mL) were exposed or not with 3 nM of T-2 toxin for 1 h before activation during 4 h at 39°C by different TLR-agonists: TLR2-agonist (HKLM); TLR4-agonist (LPS); TLR2/6-agonist (FSL1) and TLR7-agonist (Imiquimod). Relative expression of TLR genes was evaluated by quantitative real time PCR. Data were analyzed by DART-PCR software and gene expression was normalized by the mean of two housekeeping genes (RPL32 and β2-microglobuline). TLR gene expression was reported to unstimulated PAM. After checking for the normal distribution of data carried out with one-way ANOVA test, Student test was conducted to determine the differences among groups. The mean (+/− SEM) of the values obtained with PAM from five different piglets are shown. Significant differences (P < 0.05) between unstimulated cells (negative control) and cells treated by each TLR-L are marked with an asterisk (*). Significant differences (P < 0.05) between TLR-L-activated cells in the presence or not of T-2 toxin treatment are marked with (t).

ISSN: 1297-9716