Figure 4

Effects of T-2 toxin on the expression of both cell surface receptor on macrophage: TLR4 co-receptor (CD14) and mannose receptor (CD206). Cells were cultured or not for 1 h in the presence (black bars) or absence (white bars) of 3 nM of T-2 toxin before their activation with different TLR-agonists: TLR2-agonist (HKLM); TLR4-agonist (LPS); TLR2/6-agonist (FSL1) and TLR7-agonist (Imiquimod) for 16 h at 39°C, 5% CO₂. Cells were collected and CD14 (A) and CD206 (B) staining were analyzed by flow cytometry. The mean of fluorescence intensity of both of these two markers was normalized to the negative control. Mean (+/− SEM) of values obtained with PAM from five different piglets are shown. * indicates a P value < 0.05